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Electroporation and lysis of marine microalga Karenia brevis for RNA extraction and amplification

Bahi, M.M.; Tsaloglou, M-N.; Mowlem, M.; Morgan, H.. 2011 Electroporation and lysis of marine microalga Karenia brevis for RNA extraction and amplification. Journal of The Royal Society Interface, 8 (57). 601-608. 10.1098/rsif.2010.0445

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Abstract/Summary

We describe here a simple device for dielectrophoretic concentration of marine microalga Karenia brevis non-motile cells, followed by electric field-mediated lysis for RNA extraction. The lysate was purified using magnetic beads and pure RNA extracted. RNA quality was assessed off-chip by nucleic acid sequence-based amplification and the optimum conditions for lysis were determined. This procedure will form part of an integrated microfluidic system that is being developed with sub-systems for performing cell concentration and lysis, RNA extraction/purification and real-time quantitative RNA detection. The integrated system and its components could be used for a large range of applications including in situ harmful algal bloom detection, transcriptomics and point-of-care diagnostics.

Item Type: Publication - Article
Digital Object Identifier (DOI): 10.1098/rsif.2010.0445
ISSN: 1742-5662
Date made live: 13 Jan 2011 09:21 +0 (UTC)
URI: http://nora.nerc.ac.uk/id/eprint/271055

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