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Evidence for targeting common siRNA hotspots and GC preference by plant Dicer-like proteins

Ho, Thien; Wang, Hui; Pallett, Denise; Dalmay, Tamas. 2007 Evidence for targeting common siRNA hotspots and GC preference by plant Dicer-like proteins. FEBS Letters, 581 (2007). 3267-3272. 10.1016/j.febslet.2007.06.022

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Abstract/Summary

Small interfering (si)RNAs isolated from Brassica juncea leaves infected by Turnip mosaic virus (TuMV) were characterized by cloning and sequencing. The TuMV siRNA population was dominated by 21 and 22-nt long species originated mainly from the same siRNA hotspots, indicating operational similarity between the plant Dicer-like (DCL) enzymes. Robust GC bias was observed for TuMV siRNAs versus the virus genome, indicating that DCL was more likely to target GC-rich regions. Furthermore, dicot micro-(mi)RNAs displayed higher GC% than their DCL1 substrate RNAs, implicating that the GC bias may be ancient, therefore may be important for the RNAi technology.

Item Type: Publication - Article
Digital Object Identifier (DOI): 10.1016/j.febslet.2007.06.022
Programmes: CEH Programmes pre-2009 publications > Biodiversity > BD03 The Genetic Basis of Ecological Function
CEH Sections: Hails
ISSN: 0014-5793
Additional Information. Not used in RCUK Gateway to Research.: Copy in Digital Archive
Additional Keywords: siRNA, miRNA, compositional bias, Dicer, Turnip mosaic virus, Brassica juncea
NORA Subject Terms: Botany
Biology and Microbiology
Ecology and Environment
Chemistry
Date made live: 19 Mar 2008 12:26
URI: http://nora.nerc.ac.uk/id/eprint/2434

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