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Characterization and modeling of transcriptional cross-regulation in Acinetobacter baylyi ADP1

Zhang, Dayi; Zhao, Yun; He, Yi; Wang, Yun; Zhao, Yiyu; Zheng, Yi; Wei, Xia; Zhang, Litong; Li, Yuzhen; Jin, Tao; Wu, Lin; Wang, Hui; Davison, Paul A.; Xu, Junguang; Huang, Wei E.. 2012 Characterization and modeling of transcriptional cross-regulation in Acinetobacter baylyi ADP1. ACS Synthetic Biology, 1 (7). 274-283. 10.1021/sb3000244

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Abstract/Summary

Synthetic biology involves reprogramming and engineering of regulatory genes in innovative ways for the implementation of novel tasks. Transcriptional gene regulation systems induced by small molecules in prokaryotes provide a rich source for logic gates. Cross-regulation, whereby a promoter is activated by different molecules or different promoters are activated by one molecule, can be used to design an OR-gate and achieve cross-talk between gene networks in cells. Acinetobacter baylyi ADP1 is naturally transformable, readily editing its chromosomal DNA, which makes it a convenient chassis for synthetic biology. The catabolic genes for salicylate, benzoate, and catechol metabolism are located within a supraoperonic cluster (-sal-are-ben-cat-) in the chromosome of A. baylyi ADP1, which are separately regulated by LysR-type transcriptional regulators (LTTRs). ADP1-based biosensors were constructed in which salA, benA, and catB were fused with a reporter gene cassette luxCDABE under the separate control of SalR, BenM, and CatM regulators. Salicylate, benzoate, catechol, and associated metabolites were found to mediate cross-regulation among sal, ben, and cat operons. A new mathematical model was developed by considering regulator-inducer binding and promoter activation as two separate steps. This model fits the experimental data well and is shown to predict cross-regulation performance.

Item Type: Publication - Article
Digital Object Identifier (DOI): 10.1021/sb3000244
Programmes: CEH Topics & Objectives 2009 - 2012 > Biodiversity
CEH Sections: Hails
ISSN: 2161-5063
Additional Keywords: cross-regulation, Acinetobacter baylyi ADP1, catechol, salicylate, benzoate, LysR-type gene regulation, mathematic model, repressor
NORA Subject Terms: Biology and Microbiology
Date made live: 17 Jan 2013 09:52 +0 (UTC)
URI: http://nora.nerc.ac.uk/id/eprint/21186

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