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Development of microsatellite markers using 454 sequencing for the rare socially parasitic hoverfly, Microdon mutabilis

von Zum Hof, Juergen; Schonrogge, Karsten; Cook, James M.; Gardner, Michael G.. 2012 Development of microsatellite markers using 454 sequencing for the rare socially parasitic hoverfly, Microdon mutabilis. Australian Journal of Zoology, 60 (2). 108-110. 10.1071/ZO12042

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Abstract/Summary

To date there have been only limited fine-scale investigations into the molecular ecology of the European hoverfly, Microdon mutabilis, due to the paucity of available polymorphic markers.Wedescribe the development of primers amplifying five novel microsatellite loci using next-generation sequencing (454) and three previously undescribed M. mutabilis microsatellite loci using enrichments. In hoverflies from a population in Ireland, the number of alleles per locus ranged from 2 to 16, and the observed heterozygosity ranged between 0.26 and 0.97.

Item Type: Publication - Article
Digital Object Identifier (DOI): 10.1071/ZO12042
Programmes: CEH Topics & Objectives 2009 onwards > Biodiversity > BD Topic 2 - Ecological Processes in the Environment > BD - 2.3 - Identify indicators and traits of ecosystem status ...
CEH Sections: Hails
ISSN: 0004-959X
Additional Information. Not used in RCUK Gateway to Research.: This document is the author’s final manuscript version of the journal article, incorporating any revisions agreed during the peer review process. Some differences between this and the publisher’s version remain. You are advised to consult the publisher’s version if you wish to cite from this article. The definitive version is available at www.publish.csiro.au/
Additional Keywords: genetic population structure, local adaptation, PCR, social parasite
NORA Subject Terms: Ecology and Environment
Date made live: 10 Oct 2012 13:53
URI: http://nora.nerc.ac.uk/id/eprint/19884

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