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New microsatellite markers in turbot (Scophthalmus maximus) derived from an enriched genomic library and sequence databases

Pardo, G.B.; Casas, L.; Fortes, G.G.; Bouza, C.; Martinez, P.; Clark, M.S.; Sanchez, L.. 2005 New microsatellite markers in turbot (Scophthalmus maximus) derived from an enriched genomic library and sequence databases. Molecular Ecology Notes, 2005 (5). 62-64. 10.1111/j.1471-8286.2004.00834.x

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Abstract/Summary

Scophthalmus maximus is an important commercially aquaculture fish species. We tackle the search for new microsatellites using two different approaches: an enriched partial genomic library and a screening of all turbot DNA sequences deposited in GenBank. Out of 15 genomic library derived loci, five gave working primer pairs, with expected heterozygosities (HE) ranging from 0.13 to 0.91. Out of seven loci derived from database sequences, two amplified successfully with an HE ranging from 0.56 to 0.63. The average allele number of these microsatellites was 5.7 per locus with a range between two and 15.

Item Type: Publication - Article
Digital Object Identifier (DOI): 10.1111/j.1471-8286.2004.00834.x
Programmes: BAS Programmes > Antarctic Science in the Global Context (2000-2005) > Antarctic Biodiversity - Past, Present and Future
ISSN: 1471-8278
Additional Keywords: Fish, Genetics, Molecular biology
NORA Subject Terms: Zoology
Date made live: 10 Jan 2008 14:48
URI: http://nora.nerc.ac.uk/id/eprint/1904

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