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Nematode-specific PCR primers for the 18S small subunit rRNA gene

Floyd, Robin M.; Rogers, Alex D.; Lambshead, John D.; Smith, Craig R.. 2005 Nematode-specific PCR primers for the 18S small subunit rRNA gene. Molecular Ecology Notes, 5 (3). 611-612. 10.1111/j.1471-8286.2005.01009.x

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Abstract/Summary

A set of polymerase chain reaction primers were designed, which amplify a c. 1 kb fragment of the 18S ribosomal DNA gene, and are specific to the phylum Nematoda. These have proven useful in isolating nematode genes from samples mixed with other biological material, particularly with application to DNA barcoding. Optimal reaction conditions are described. These primers have successfully amplified the correct fragment from a wide phylogenetic range of nematodes, and have so far generated no sequences from any other organismal group.

Item Type: Publication - Article
Digital Object Identifier (DOI): 10.1111/j.1471-8286.2005.01009.x
Programmes: BAS Programmes > Antarctic Science in the Global Context (2000-2005) > Antarctic Biodiversity - Past, Present and Future
ISSN: 1471-8278
Additional Keywords: Nematodes, Polymerase chain reaction, DNA barcoding
NORA Subject Terms: Zoology
Biology and Microbiology
Date made live: 24 Dec 2007 12:12
URI: http://nora.nerc.ac.uk/id/eprint/1765

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