Nematode-specific PCR primers for the 18S small subunit rRNA gene
Floyd, Robin M.; Rogers, Alex D.; Lambshead, John D.; Smith, Craig R.. 2005 Nematode-specific PCR primers for the 18S small subunit rRNA gene. Molecular Ecology Notes, 5 (3). 611-612. doi: 10.1111/j.1471-8286.2005.01009.xFull text not available from this repository.
A set of polymerase chain reaction primers were designed, which amplify a c. 1 kb fragment of the 18S ribosomal DNA gene, and are specific to the phylum Nematoda. These have proven useful in isolating nematode genes from samples mixed with other biological material, particularly with application to DNA barcoding. Optimal reaction conditions are described. These primers have successfully amplified the correct fragment from a wide phylogenetic range of nematodes, and have so far generated no sequences from any other organismal group.
|Identification Number/DOI:||doi: 10.1111/j.1471-8286.2005.01009.x|
|Programmes:||BAS Programmes > Antarctic Science in the Global Context (2000-2005) > Antarctic Biodiversity - Past, Present and Future|
|Additional Keywords:||Nematodes, Polymerase chain reaction, DNA barcoding|
|NORA Subject Terms:||Zoology
Biology and Microbiology
|Date made live:||24 Dec 2007 12:12|
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