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Salivary fluid secretion in the ixodid tick Rhipicephalus appendiculatus is inhibited by Thogoto virus infection

Kaufman, W. Reuben; Bowman, Alan S.; Nuttall, Patricia A.. 2001 Salivary fluid secretion in the ixodid tick Rhipicephalus appendiculatus is inhibited by Thogoto virus infection. Experimental and Applied Acarology, 25 (8). 661-674. 10.1023/A:1016184101160

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Abstract/Summary

Adult Rhipicephalus appendiculatus ticks, infectedwith Thogoto (THO) virus or control, were fed on guinea pigs and removed atintervals throughout the feeding cycle. Salivary fluid secretion was measuredbyan in vitro technique. The salivary glandsof infected, partially-fed ticks secreted fluid in vitro at about 75% the rateof controls, but the difference between infected and controls among engorgedticks was not statistically significant. Basal and DA-stimulated levels ofcyclic AMP (cAMP) were determined in isolated glands and were significantlyaffected by THO virus infection. The differences in secretory rate amongcontroland infected ticks could not be explained in terms of altered cAMP levels.Haemolymph volume was measured by a tracer-dilution technique using3H-inulin. The mean haemolymph volume for both THO-infected andcontrol groups was between 23–24% body weight throughout the feedingcycle, indicating that infection by this arbovirus did not influence salivaryfluid secretion via altered haemolymph volume. The mechanism by which THO virusaffects secretory activity of its tick vector remains unknown

Item Type: Publication - Article
Digital Object Identifier (DOI): 10.1023/A:1016184101160
Programmes: CEH Programmes pre-2009 publications > Other
CEH Sections: _ Pre-2000 sections
ISSN: 0168-8162
Additional Keywords: Arbovirus, cyclic AMP, Rhipicephalus appendiculatus, Thogoto virus, tick salivary glands
NORA Subject Terms: Biology and Microbiology
Date made live: 02 Oct 2012 11:07
URI: http://nora.nerc.ac.uk/id/eprint/17500

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