Proteomics of cryoprotective dehydration in Megaphorura arctica Tullberg 1876 (Onychiuridae: Collembola)
Thorne, M.A.S.; Worland, M.R.; Feret, R.; Deery, M.J.; Lilley, K.S.; Clark, Melody. 2011 Proteomics of cryoprotective dehydration in Megaphorura arctica Tullberg 1876 (Onychiuridae: Collembola). Insect Molecular Biology, 20 (3). 303-310. 10.1111/j.1365-2583.2010.01062.xFull text not available from this repository.
The Arctic springtail, Megaphorura arctica Tullberg 1876 (Onychiuridae: Collembola), is one of the few organisms known to survive the extreme stresses of its environment by using cryoprotective dehydration. We have undertaken a proteomics study comparing M. arctica, acclimated at -2 degrees C, the temperature known to induce the production of the anhydroprotectant trehalose in this species, and -6 degrees C, the temperature at which trehalose expression plateaus, against control animals acclimated at +5 degrees C. Using difference gel electrophoresis, and liquid chromatography tandem mass spectrometry, we identified three categories of differentially expressed proteins with specific functions, up-regulated in both the -2 degrees C and -6 degrees C animals, that were involved in metabolism, membrane transport and protein folding. Proteins involved in cytoskeleton organisation were only up-regulated in the -6 degrees C animals.
|Programmes:||BAS Programmes > Polar Science for Planet Earth (2009 - ) > Ecosystems|
|Additional Keywords:||cold tolerance, DiGE, LC-MS/MS, Onychiurus arcticus, springtail|
|Date made live:||07 Feb 2012 13:52|
Actions (login required)