Baculovirus transfer vectors
Possee, Robert D.; King, Linda A.. 2007 Baculovirus transfer vectors. In: Murhammer, D.W., (ed.) Baculovirus and insect cell expression protocols. 2nd edition. Totowa, New Jersey, USA, Humana Press, 55-76. (Methods in Molecular Biology, 388).Full text not available from this repository.
The production of a recombinant baculovirus expression vector normally involves mixing infectious virus DNA with a plasmid-based transfer vector and then cotransfecting insect cells to initiate virus infection. The aim of this chapter is to provide an update on the range of baculovirus transfer vectors currently available. It is impractical to list every transfer vector that has ever been used. Instead, we focus on those that are available commercially and should be easy to locate. These vectors permit the insertion of single or multiple genes for expression, or the production of proteins with specific peptide tags that aid subsequent protein purification. A table listing the transfer vectors also included information on the parental virus that should be used with each one. Recent developments in recombinant baculovirus production are also described. Some of these permit the direct insertion of a recombinant gene into the virus genome without the requirement for a transfer vector. The information provided should enable new users of the system to choose those reagents most suitable for their purposes.
|Item Type:||Book Section|
|Programmes:||CEH Programmes pre-2009 publications > Biodiversity|
|Additional Keywords:||Baculovirus, polyhedrin gene, p10 gene, single/multiple expression vectors, fusion vectors, biotechnology, molecular biology, virology|
|NORA Subject Terms:||Biology and Microbiology|
|Date made live:||14 Dec 2007 10:04|
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