Rapid method for coextraction of DNA and RNA from natural environments for analysis of ribosomal DNA- and rRNA-based microbial community composition
Griffiths, Robert I.; Whiteley, Andrew S.; O'Donnell, Anthony G.; Bailey, Mark J.. 2000 Rapid method for coextraction of DNA and RNA from natural environments for analysis of ribosomal DNA- and rRNA-based microbial community composition. Applied and Environmental Microbiology, 66 (12). 5488-5491.Full text not available from this repository.
A rapid protocol for the extraction of total nucleic acids from environmental samples is described. The method facilitates concomitant assessment of microbial 16S rRNA diversity by PCR and reverse transcription- PCR amplification from a single extraction. Denaturing gradient gel electrophoresis microbial community analysis differentiated the active component (rRNA derived) from the total bacterial diversity (ribosomal DNA derived) down the horizons of an established grassland soil.
|Programmes:||CEH Programmes pre-2009 publications > Biodiversity > BD03 The Genetic Basis of Ecological Function|
|CEH Sections:||_ Pre-2000 sections|
|NORA Subject Terms:||Biology and Microbiology|
|Date made live:||23 Aug 2011 08:34|
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