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Partitioning core and satellite taxa from within cystic fibrosis lung bacterial communities

van der Gast, Christopher J.; Walker, Alan W.; Stressmann, Franziska A.; Rogers, Geraint B.; Scott, Paul; Daniels, Thomas W.; Carroll, Mary P.; Parkhill, Julian; Bruce, Kenneth D.. 2011 Partitioning core and satellite taxa from within cystic fibrosis lung bacterial communities. ISME Journal, 5. 780-791. 10.1038/ismej.2010.175

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Abstract/Summary

Cystic fibrosis (CF) patients suffer from chronic bacterial lung infections that lead to death in the majority of cases. The need to maintain lung function in these patients means that characterising these infections is vital. Increasingly, culture-independent analyses are expanding the number of bacterial species associated with CF respiratory samples; however, the potential significance of these species is not known. Here, we applied ecological statistical tools to such culture-independent data, in a novel manner, to partition taxa within the metacommunity into core and satellite species. Sputa and clinical data were obtained from 14 clinically stable adult CF patients. Fourteen rRNA gene libraries were constructed with 35 genera and 82 taxa, identified in 2139 bacterial clones. Shannon–Wiener and taxa-richness analyses confirmed no undersampling of bacterial diversity. By decomposing the distribution using the ratio of variance to the mean taxon abundance, we partitioned objectively the species abundance distribution into core and satellite species. The satellite group comprised 67 bacterial taxa from 33 genera and the core group, 15 taxa from 7 genera (including Pseudomonas (1 taxon), Streptococcus (2), Neisseria (2), Catonella (1), Porphyromonas (1), Prevotella (5) and Veillonella (3)], the last four being anaerobes). The core group was dominated by Pseudomonas aeruginosa. Other recognised CF pathogens were rare. Mantel and partial Mantel tests assessed which clinical factors influenced the composition observed. CF transmembrane conductance regulator genotype and antibiotic treatment correlated with all core taxa. Lung function correlated with richness. The clinical significance of these core and satellite species findings in the CF lung is discussed.

Item Type: Publication - Article
Digital Object Identifier (DOI): 10.1038/ismej.2010.175
Programmes: CEH Topics & Objectives 2009 onwards > Biodiversity > BD Topic 1 - Observations, Patterns, and Predictions for Biodiversity
CEH Sections: Hails
ISSN: 1751-7362
Additional Information. Not used in RCUK Gateway to Research.: This article is available as Open Access. Please click on the OFFICIAL URL link to access full text
Additional Keywords: commonness and rarity, oral microbiota, polymicrobial infections, pulmonary disease, species abundance distributions
NORA Subject Terms: Biology and Microbiology
Date made live: 26 May 2011 09:07
URI: http://nora.nerc.ac.uk/id/eprint/13434

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