Functionalisation of whole-cell bacterial reporters with magnetic nanoparticles
Zhang, Dayi; Fakhrullin, Rawil F.; Ozmen, Mustafa; Wang, Hui; Wang, Jian; Paunov, Vesselin N.; Li, Guanghe; Huang, Wei E.. 2011 Functionalisation of whole-cell bacterial reporters with magnetic nanoparticles. Microbial Biotechnology, 4 (1). 89-97. 10.1111/j.1751-7915.2010.00228.xFull text not available from this repository.
We developed a biocompatible and highly efficient approach for functionalization of bacterial cell wall with magnetic nanoparticles (MNPs). Three Acinetobacter baylyi ADP1 chromosomally based bioreporters, which were genetically engineered to express bioluminescence in response to salicylate, toluene/ xylene and alkanes, were functionalized with 18 3 nm iron oxide MNPs to acquire magnetic function. The efficiency of MNPs functionalization of Acinetobacter bioreporters was 99.96 0.01%. The MNPs-functionalized bioreporters (MFBs) can be remotely controlled and collected by an external magnetic field. The MFBs were all viable and functional as good as the native cells in terms of sensitivity, specificity and quantitative response. More importantly, we demonstrated that salicylate sensing MFBs can be applied to sediments and garden soils, and semiquantitatively detect salicylate in those samples by discriminably recovering MFBs with a permanent magnet. The magnetically functionalized cells are especially useful to complex environments in which the indigenous cells, particles and impurities may interfere with direct measurement of bioreporter cells and conventional filtration is not applicable to distinguish and harvest bioreporters. The approach described here provides a powerful tool to remotely control and selectively manipulate MNPsfunctionalized cells in water and soils. It would have a potential in the application of environmental microbiology, such as bioremediation enhancement and environment monitoring and assessment.
|Programmes:||CEH Topics & Objectives 2009 onwards > Biodiversity > BD Topic 2 - Ecological Processes in the Environment > BD - 2.4 - Estimate the impact of the main drivers and pressures on biodiversity ...|
|NORA Subject Terms:||Biology and Microbiology|
|Date made live:||10 Mar 2011 16:23|
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