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Nucleotide bias of DCL and AGO in plant anti-virus gene silencing

Ho, Thien; Wang, Liang; Huang, Linfeng; Li, Zhigang; Pallett, Denise W.; Dalmay, Tamas; Ohshima, Kazusato; Walsh, John A.; Wang, Hui. 2010 Nucleotide bias of DCL and AGO in plant anti-virus gene silencing. Protein & Cell, 1. 847-858. 10.1007/s13238-010-0100-4

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Abstract/Summary

Plant Dicer-like (DCL) and Argonaute (AGO) are the key enzymes involved in anti-virus post-transcriptional gene silencing (AV-PTGS). Here we show that AV-PTGS exhibited nucleotide preference by calculating a relative AV-PTGS efficiency on processing viral RNA substrates. In comparison with genome sequences of dicot-infecting Turnip mosaic virus (TuMV) and monocot-infecting Cocksfoot streak virus (CSV), viral-derived small interfering RNAs (vsiRNAs) displayed positive correlations between AV-PTGS efficiency and G+C content (GC%). Further investigations on nucleotide contents revealed that the vsiRNA populations had G-biases. This finding was further supported by our analyses of previously reported vsiRNA populations in diverse plant-virus associations, and AGO associated Arabidopsis endogenous siRNA populations, indicating that plant AGOs operated with G-preference. We further propose a hypothesis that AV-PTGS imposes selection pressure(s) on the evolution of plant viruses. This hypothesis was supported when potyvirus genomes were analysed for evidence of GC elimination, suggesting that plant virus evolution to have low GC% genomes would have a unique function, which is to reduce the host AV-PTGS attack during infections.

Item Type: Publication - Article
Digital Object Identifier (DOI): 10.1007/s13238-010-0100-4
Programmes: CEH Topics & Objectives 2009 onwards > Biodiversity > BD Topic 2 - Ecological Processes in the Environment > BD - 2.2 - Quantify the impact of invasive species, pathogens ...
CEH Sections: Hails
ISSN: 1674-800X
NORA Subject Terms: Biology and Microbiology
Date made live: 25 Jan 2011 14:49
URI: http://nora.nerc.ac.uk/id/eprint/13126

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