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Role of antioxidant defense during different stages of preadult life cycle in European corn borer (Ostrinia nubilalis, Hubn.): diapause and metamorphosis

Jovanović-Galović, A.; Blagojević, D.P.; Grubor-Lajšić, G.; Worland, R.; Spasić, M.B.. 2004 Role of antioxidant defense during different stages of preadult life cycle in European corn borer (Ostrinia nubilalis, Hubn.): diapause and metamorphosis. Archives of Insect Biochemistry and Physiology, 55 (2). 79-89. 10.1002/arch.10126

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Abstract/Summary

Antioxidant enzymes, total glutathione (GSH), and ascorbic acid (ASA) were determined in whole body homogenates of nondiapausing larvae, diapausing larvae during the diapausing period (October, December, and February), and in pupae emerged from both diapausing and nondiapausing larvae of the European corn borer (Ostrinia nubilalis, Hubn., Lepidoptera: Pyralidae). The activities of catalase, selenium nondependent glutathione peroxidase (GPx), and glutathione-S-transferase (GST), as well as the content of GSH and ASA, were found to vary throughout the larval diapause. Compared to diapausing larvae, nondiapausing larvae were higher in levels of catalose, GPx, GST, and dehydroascorbate reductase (DHAR) activity. GSH content was also increased. However, nondiapausing larvae contained less ASA than diapausing ones. Pupae had higher GPx and GST activity and an increased ASA content compared to larvae. The pupae emerged from nondiapausing larvae had higher GST, glutathione reductase (GR), and DHAR activities, but lower GPx activity and ASA content than those emerged from diapausing larvae. Correlation analysis revealed differences in the way the antioxidant level is equilibrated for a particular stage and developmental pattern. The results suggest that cellular antioxidants are involved in both the protection of cells and the regulation of redox levels during the pre-adult stages of Ostrinia nubilalis.

Item Type: Publication - Article
Digital Object Identifier (DOI): 10.1002/arch.10126
Programmes: BAS Programmes > Antarctic Science in the Global Context (2000-2005) > Life at the Edge - Stresses and Thresholds
ISSN: 0739-4462
Additional Keywords: Ostrinia, diapause, larvae, pupae, glutathione, ascorbate, catalase
NORA Subject Terms: Biology and Microbiology
Ecology and Environment
Chemistry
Date made live: 18 Jan 2012 15:39
URI: http://nora.nerc.ac.uk/id/eprint/12216

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