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Detection of QTL with effects on osmoregulation capacities in the rainbow trout (Oncorhynchus mykiss)

Le Bras, Yvan; Dechamp, Nicolas; Krieg, Francine; Filangi, Olivier; Guyomard, Rene; Boussaha, Mekki; Bovenhuis, Henk; Pottinger, Thomas G.; Prunet, Patrick; Le Roy, Pascale; Quillet, Edwige. 2011 Detection of QTL with effects on osmoregulation capacities in the rainbow trout (Oncorhynchus mykiss). BMC Genetics, 12, 46. 10.1186/1471-2156-12-46

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Abstract/Summary

Background There is increasing evidence that the ability to adapt to seawater in teleost fish is modulated by genetic factors. Most studies have involved the comparison of species or strains and little is known about the genetic architecture of the trait. To address this question, we searched for QTL affecting osmoregulation capacities after transfer to saline water in a nonmigratory captive-bred population of rainbow trout. Results A QTL design (5 full-sib families, about 200 F2 progeny each) was produced from a cross between F0 grand-parents previously selected during two generations for a high or a low cortisol response after a standardized confinement stress. When fish were about 18 months old (204 g body weight), individual progeny were submitted to two successive hyper-osmotic challenges (30g of salt/L) at a 14 d interval. Plasma chloride and sodium concentrations were recorded 24h after each transfer. After the second challenge, fish were sacrificed and gill index (weight of total gill arches corrected for body weight) was recorded. The genome scan was performed using 200 microsatellites and 88 SNP markers. Unitrait and multitrait QTL analyses evidenced a total of 15 and 7 different QTL (P<0.10) for plasma ion concentrations and gill index respectively. Among the most significant QTL, three affected concentrations of both chloride and sodium during both challenges, two were specific to either chloride or sodium concentrations, three QTL were specific to gill index, and three affected both gill index and ionic concentrations in plasma. Altogether, allelic effects were consistent for QTL affecting chloride and sodium concentrations but inconsistent for QTL affecting ionic concentrations and gill morphology. There was no systematic lineage effect (grand-parental origin of QTL alleles) on the recorded traits. Conclusions For the first time, genomic loci associated with effects on major physiological components of osmotic adaptation to seawater in a nonmigratory fish were revealed. The results pave the way for further deciphering of the complex regulatory mechanisms underlying seawater adaptation and genes involved in osmoregulatory physiology in rainbow trout and other euryhaline fishes.

Item Type: Publication - Article
Digital Object Identifier (DOI): 10.1186/1471-2156-12-46
Programmes: CEH Topics & Objectives 2009 - 2012 > Water > WA Topic 2 - Ecohydrological Processes
CEH Sections: Parr
ISSN: 1471-2156
Additional Information. Not used in RCUK Gateway to Research.: BMC Genetics is an Open Access journal. To access full text of published version, please click on the OFFICIAL URL link
NORA Subject Terms: Zoology
Biology and Microbiology
Ecology and Environment
Date made live: 23 Jun 2011 11:31
URI: http://nora.nerc.ac.uk/id/eprint/11420

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