Can genetic bar-coding be used to identify aquatic Ranunculus L. subgenus Batrachium (DC) A. Gray? A test using some species from the British Isles

Telford, Annika; O'Hare, Matthew T.; Cavers, Stephen; Holmes, Nigel. 2011 Can genetic bar-coding be used to identify aquatic Ranunculus L. subgenus Batrachium (DC) A. Gray? A test using some species from the British Isles. Aquatic Botany, 95 (1). 65-70. 10.1016/j.aquabot.2011.03.004

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Abstract/Summary

Aquatic Batrachium Ranunculus species are a key component of river macrophyte communities selected for protection under European Union legislation. The group’s simplified morphology and variable taxonomic interpretation often makes identification to species level very difficult. A genetic approach was trialled as an alternative, more reliable, means of identification. DNA barcoding using four markers (chloroplast and nuclear) was tested. The chloroplast sequence trnH-psbA worked best and allowed identification of three out of five species while nuclear sequences supported the identification of two hybrids. This method is amenable to simplification through techniques such as PCR-RFLP or RT-PCR. It has the potential to provide easy, rapid and inexpensive identification of Batrachium Ranunculus species.

Item Type:	Article
Identification Number/DOI:	10.1016/j.aquabot.2011.03.004
Programmes:	CEH Topics & Objectives 2009 onwards > Biodiversity > BD Topic 2 - Ecological Processes in the Environment > BD - 2.3 - Identify indicators and traits of ecosystem status ...
CEH Sections:	Watt
ISSN:	0304-3770
Additional Keywords:	Ranunculus, Batrachium,DNA barcoding, hybrid, Internal transcribed spacer(ITS), matK, trnC-ycf6, trnH-psbA
NORA Subject Terms:	Biology and Microbiology Ecology and Environment
Date made live:	25 May 2011 08:55
URI:	http://nora.nerc.ac.uk/id/eprint/11286

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