Detection of fungal 18S rRNA sequences in conjunction with marine nematode 18S rRNA amplicons
Bhadury, Punyasloke; Bridge, Paul D.; Austen, Melanie C.; Bilton, David T.; Smerdon, Gary R.. 2009 Detection of fungal 18S rRNA sequences in conjunction with marine nematode 18S rRNA amplicons. Aquatic Biology, 5 (2). 149-155. 10.3354/ab00145Full text not available from this repository.
Free-living nematodes constitute an important component of estuarine and marine benthic ecosystems. Some marine and soil nematodes are known to harbor microbes, including symbiotic bacteria and fungi, in their external cuticle as well as internally. While assessing diversity of marine nematodes from southwest England using molecular approaches, we found evidence of co-amplification of fungal 18S rRNA sequences in conjunction with nematode 18S rRNA sequences. Based on an 18S rRNA PCR-DGGE approach, 3 fungal clone types were detected alongside nematodes from 2 of 4 estuarine and marine sites in southwest England. At the phylogeny level, fungal clone type 1 belongs to Chaetothyriales while the other 2 clone types belong to Hypocreales. The fungal clones were co-amplified with specific marine nematode taxa indicating true ecological association rather than transient environmental contamination. The present study is the first to detect fungal 18S sequences in parallel with marine nematodes and opens up a new avenue of research for investigating ecological interactions between nematodes and fungi in the marine environment.
|Item Type:||Publication - Article|
|Digital Object Identifier (DOI):||10.3354/ab00145|
|Programmes:||BAS Programmes > Global Science in the Antarctic Context (2005-2009) > Long-Term Monitoring and Survey – Biological Sciences Division|
|Additional Keywords:||marine nematodes; 18SrRNA; DGGE; fungi|
|NORA Subject Terms:||Marine Sciences
Biology and Microbiology
Ecology and Environment
|Date made live:||18 Oct 2010 10:34|
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