Raman FISH

Read, D.S.; Huang, W.E.; Whiteley, A.S.. 2009 Raman FISH. In: Timmis, Kenneth. N.; McGenity, Terry; Meer, Jan Roelof van der; Lorenzo, Victor de, (eds.) Handbook of Hydrocarbon and Lipid Microbiology. Springer, 4027-4038.

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Linking both identity and function within natural microbial communities has long been seen as one of the ultimate goals for microbial ecologists. The phenotypic data obtained from single-cell Raman microspectroscopy can be combined with the phylogenetic resolution of fluorescence in situ hybridization (FISH) to provide a powerful tool for investigating the structure and function of natural microbial communities (Raman-FISH). This chapter provides information about the use of isotopically labeled substrates to track isotope incorporation and hence specific metabolic processes within bacterial functional groups, through the use of Raman-FISH. A general overview of the equipment and techniques necessary for conducting Raman-FISH analysis is followed by a case study highlighting the utility of this technique for discovering the bacterial communities responsible for naphthalene degradation in groundwater microcosms.

Item Type: Publication - Book Section
Digital Object Identifier (DOI): 10.1007/978-3-540-77587-4
Programmes: CEH Topics & Objectives 2009 - 2012 > Biodiversity > BD Topic 2 - Ecological Processes in the Environment > BD - 2.1 - Interactions ... structure ecosystems and their functioning
CEH Programmes pre-2009 publications > Biodiversity > BD03 The Genetic Basis of Ecological Function > BD03.1 Molecular ecology
CEH Sections: Hails
ISBN: 978-3-540-77588-1
Additional Keywords: fluorescence in situ hybridization, raman spectroscopy, stable isotopes
NORA Subject Terms: Biology and Microbiology
Related URLs:
Date made live: 13 Oct 2010 13:19

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