Read, D.S.; Huang, W.E.; Whiteley, A.S.. 2009 Raman FISH. In: Timmis, Kenneth. N.; McGenity, Terry; Meer, Jan Roelof van der; Lorenzo, Victor de, (eds.) Handbook of Hydrocarbon and Lipid Microbiology. Springer, 4027-4038.Full text not available from this repository.
Linking both identity and function within natural microbial communities has long been seen as one of the ultimate goals for microbial ecologists. The phenotypic data obtained from single-cell Raman microspectroscopy can be combined with the phylogenetic resolution of fluorescence in situ hybridization (FISH) to provide a powerful tool for investigating the structure and function of natural microbial communities (Raman-FISH). This chapter provides information about the use of isotopically labeled substrates to track isotope incorporation and hence specific metabolic processes within bacterial functional groups, through the use of Raman-FISH. A general overview of the equipment and techniques necessary for conducting Raman-FISH analysis is followed by a case study highlighting the utility of this technique for discovering the bacterial communities responsible for naphthalene degradation in groundwater microcosms.
|Item Type:||Publication - Book Section|
|Digital Object Identifier (DOI):||10.1007/978-3-540-77587-4|
|Programmes:||CEH Topics & Objectives 2009 onwards > Biodiversity > BD Topic 2 - Ecological Processes in the Environment > BD - 2.1 - Interactions ... structure ecosystems and their functioning
CEH Programmes pre-2009 publications > Biodiversity > BD03 The Genetic Basis of Ecological Function > BD03.1 Molecular ecology
|Additional Keywords:||fluorescence in situ hybridization, raman spectroscopy, stable isotopes|
|NORA Subject Terms:||Biology and Microbiology|
|Date made live:||13 Oct 2010 13:19|
Actions (login required)