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Assessment of Calanus finmarchicus growth and dormancy using the aminoacyl-tRNA synthetases method

Yebra, L.; Hirst, A.G.; Hernandez-Leon, S.. 2006 Assessment of Calanus finmarchicus growth and dormancy using the aminoacyl-tRNA synthetases method. Journal of Plankton Research, 28 (12). 1191-1198. 10.1093/plankt/fbl049

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Abstract/Summary

We obtained growth rates of the copepod Calanus finmarchicus at different locations across the North Atlantic between May 1998 and June 2004. Animals were incubated for 2–9 days and fed either with natural food assemblages or with cultured algae. During this period, we measured both somatic weight-specific growth rates (measured as protein change) and aminoacyl-tRNA synthetases (AARS) activity. We found a highly significant relationship between AARS activity and growth in protein content (R2 = 0.55, P < 0.001). Significant AARS activity also occurred when growth was negative, the relationship predicting an AARS activity level of 8.33 nmPPi·mg protein–1·h–1 when somatic growth is zero. This is because AARS activity is expected even when growth is negative, owing to the continued protein turnover in the cells. The AARS method allowed for the first time the study of protein metabolism in overwintering C. finmarchicus. Our study results showed that overwintering copepods had significantly lower values of AARS activity than non-diapausing animals (t = –3.51, P < 0.002). The AARS method opens the possibility to better understand physiology dynamics of deep-water organisms (e.g. the beginning and end of diapause).

Item Type: Publication - Article
Digital Object Identifier (DOI): 10.1093/plankt/fbl049
Programmes: BAS Programmes > Global Science in the Antarctic Context (2005-2009) > DISCOVERY 2010 - Integrating Southern Ocean Ecosystems into the Earth System
ISSN: 0142-7873
Additional Information. Not used in RCUK Gateway to Research.: Full text not available from this repository
Additional Keywords: Copepods, Growth rates
NORA Subject Terms: Zoology
Date made live: 05 Oct 2007 13:32
URI: http://nora.nerc.ac.uk/id/eprint/1051

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