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Polyphasic study of Antarctic cyanobacterial strains

Taton, Arnaud; Grubisic, Stana; Ertz, Damien; Hodgson, Dominic A.; Piccardi, Raffaella; Biondi, Natascia; Tredici, Mario R.; Mainini, Mariangela; Losi, Daniele; Marinelli, Flavia; Wilmotte, Annick. 2006 Polyphasic study of Antarctic cyanobacterial strains. Journal of Phycology, 42 (6). 1257-1270. 10.1111/j.1529-8817.2006.00278.x

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Abstract/Summary

We isolated 59 strains of cyanobacteria from the benthic microbial mats of 23 Antarctic lakes, from five locations in two regions, in order to characterize their morphological and genotypic diversity. On the basis of their morphology, the cyanobacteria were assigned to 12 species that included four Antarctic endemic taxa. Sequences of the ribosomal RNA gene were determined for 56 strains. In general, the strains closely related at the 16S rRNA gene level belonged to the same morphospecies. Nevertheless, divergences were observed concerning the diversity in terms of species richness, novelty, and geographical distribution. For the 56 strains, 21 operational taxonomic units (OTUs, defined as groups of partial 16S rRNA gene sequences with more than 97.5% similarity) were found, including nine novel and three exclusively Antarctic OTUs. Sequences of Petalonema cf. involvens and Chondrocystis sp. were determined for the first time. The internally transcribed spacer (ITS) between the 16S and the 23S rRNA genes was sequenced for 33 strains, and similar groupings were observed with the 16S rRNA gene and the ITS, even when the strains were derived from different lakes and regions. In addition, 48 strains were screened for antimicrobial and cytotoxic activities, and 17 strains were bioactive against the gram-positive Staphylococcus aureus, or the fungi Aspergillus fumigatus and Cryptococcus neoformans. The bioactivities were not in coincidence with the phylogenetic relationships, but rather were specific to certain strains.

Item Type: Publication - Article
Digital Object Identifier (DOI): 10.1111/j.1529-8817.2006.00278.x
Programmes: BAS Programmes > Global Science in the Antarctic Context (2005-2009) > Climate and Chemistry - Forcings and Phasings in the Earth System
ISSN: 0022-3646
Additional Information. Not used in RCUK Gateway to Research.: Full text not available from this repository
Additional Keywords: Cyanobacteria, lakes, microbial mats, rRNA
NORA Subject Terms: Biology and Microbiology
Date made live: 04 Oct 2007 16:20
URI: http://nora.nerc.ac.uk/id/eprint/1042

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